Phytopathologic, Histologic and Molecular Characterizacion of the Interaction Gluconacetobacter Diazotrophicus-Xanthomonas Albilineans-Sugarcane
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Abstract
Searching for resistance to diseases by different methods is of special importance regarding strategies to reach a sustainable sugarcane agriculture. In vitro studies in this sense have reported the potential of the bacterium Gluconacetobacter diazotrophicus (Gd) as antagonist of Xanthomonas albilineans (Xa), bacterium that causes leaf scald in sugarcane. The aim of this work was the phytopathologic, histologic and molecular characterization of the endophyte-sugarcane-pathogen interaction. To reach this objective, cultivars C1051-73 and C90-469 were selected with four treatments: healthy control, Xa inoculation, Gd inoculation and Gd+Xa inoculation. Samples were taken monthly and the number of plants of each cultivar showing symptoms per treatments and replicates was registered up to four months after inoculations. The presence of each bacterium was detected by specific PCR and the histologic changes by means of transversal cuts. The pathogenicity of Xa and the effectiveness of the inoculations was demonstrated by leaf scald typical symptoms development in the susceptible control cultivar C1051-73. In the rest of treatments Xa was not detected which coincide with the accumulation of a refracting substance in the parenchimatic cells surrounding the vascular vessels. The action of Gd over Xa may have been the cause that Xa could not be settled which is in agreement with the intensity decrease of the DNA specific fragment amplified by PCR.
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