PHOTORHABDUS LUMINESCENS SYMBIONT OF THE ENTOMOPATOGENIC NEMATODE HETERORHABDITIS AMAZONENSIS
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Abstract
Photorhabdus luminescens is an enteric bacterium, that lives in symbiotic association with soil nematodes and is highly pathogenic for a wide number of insects. The bacterium is transported in the digestive tract of the nematode and once it enters the host insect through natural openings (mouth, anus and spiracles), it regurgitates the bacteria in the hemolymph of the insect, which dies from sepsis between 24-48 hours. The objective of the work was to isolate the Photorhabdus luminescens bacteria from the nematode Heterorhabditis amazonensis. The isolations were carried out by sowing the macerate of Galleria mellonella larvae previously inoculated with the nematode, in Petri dishes with NBTA culture medium. The morphological and biochemical characterization of the selected colonies was carried out; as well as in vivo tests to determine the pathogenicity of the bacteria on G. mellonella larvae. The colonies obtained presented the characteristic bioluminescence of the bacterial species, when subjected to the action of ultraviolet light. Small to medium-sized colonies were observed, with regular, convex, shiny, greenish color edges that gradually turn red. The greatest mortality of the larvae occurs between 48 to 72 hours of exposure to the bacteria.
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