HISTOLOGY OF IN VITRO SUGARCANE (Saccharum spp.) SHOOTS IN TEMPORARY IMMERSION BIOREACTORS.
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Abstract
During the in vitro propagation of sugarcane (Saccharum spp.), initially the presence of phenols was considered an undesirable trait, because their oxidation inhibits the development of the explant and caused its death. However, in the Temporary Immersion Bioreactors it was determined that the phenolic compounds in the liquid culture medium did not affect the in vitro multiplication of the shoots. The present work wasdeveloped with the objective of analyzing the histological characterization of leaves from in vitro sugarcane shoots multiplied in Temporary Immersion Bioreactors with the presence and absence of phenolic compounds in the culture medium. Ten 1.0 cm long samples were taking from the middle portion of the leaves of the in vitro shoots of the C1051-73 cultivar at seven, 14, and 21 days of culture. The results obtained demonstrate the association between the structural and biochemical changes resulting from the excretion of phenolic compounds to the culture medium by the in vitro shoots.
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