Diagnosis of Xanthomonas albilineans (Ashby) Dowson by Tissue Blot Immunosorbent Assay
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Abstract
Leaf scald is one of the major diseases of sugarcane in the world. It causes severe losses in both agricultural and industrial yields. During years, visual analysis was the mechanisms for disease diagnostic. Achieve of this paper was to apply the tissue impression immunological technique (TBIA) for the diagnosis of X. albilineans. Leaves of susceptible cultivars Co3-551, C90-176 y PR980 were positive controls, while C86-56 was the negative, confirmed by PCR (polymerase chain reaction) using specifics primers for Xa. For TBIA the leaves were press over a nitrocellulose membrane and leaf dried at room temperature. Different dilutions and combination of the specifics antiserum anti-mouse-Xanthomonas albilineans (Xalb) and anti-mouse for Xalb conjugated with alkaline phosphatase from “agdia” were used. Dark blue stain color was showed over the nitrocellulose membranes where the vascular bundles were imprinted in susceptible cultivars, while no color was observed in negative control. Best results were observed with the specifics antiserum anti-Xalb diluted 1/1500 and the anti-mouse for Xalb conjugated with alkaline phosphatase diluted 1/3500.
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